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sciflexarrayer sx microarray spotter  (SCIENION)


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    Structured Review

    SCIENION sciflexarrayer sx microarray spotter
    Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of <t>microarray-based</t> proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.
    Sciflexarrayer Sx Microarray Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+sx+microarray+spotter/sciFLEXARRAYER+SX/10__1002_slash_adfm__202315035-450-19-23
    Average 95 stars, based on 48 article reviews
    sciflexarrayer sx microarray spotter - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices"

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices

    Journal: Advanced Functional Materials

    doi: 10.1002/adfm.202315035

    Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.
    Figure Legend Snippet: Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.

    Techniques Used: Confocal Microscopy, Cell Culture, Migration, Microarray, Concentration Assay, Co-Culture Assay

    Related Articles

    Microarray:

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices
    Article Snippet: Moist tissues wetted with sterile water were placed within the well plates containing the OoC to prevent evaporation of the cell- and spheroid-embedded Matrigel solutions throughout the seeding process. .. Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion). .. Anti-CXCL12 antibodies (Cat# MAB350, R&D Systems) were diluted in spotting buffer containing 15% 2,3-butanediol and 1 m betaine in PBS to achieve a final concentration of 100 μg mL−1.

    Article Title: Nanoporous PEGDA ink for High-Resolution Additive Manufacturing of Scaffolds for Organ-on-a-Chip
    Article Snippet: Moist tissues wettened with sterile water were placed within the well plates containing the OoC to prevent evaporation of the cell- and spheroid-embedded Matrigel solutions throughout the seeding process. .. The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion). .. Anti-CXCL12 antibodies (Cat# MAB350, R&D Systems) were diluted in spotting buffer containing 15% 2,3-butanediol and 1 M betaine in PBS to achieve a final concentration of 100 μg/mL.



    Similar Products

    sciflexarrayer sx microarray spotter  (SCIENION)
    95
    SCIENION sciflexarrayer sx microarray spotter
    Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of <t>microarray-based</t> proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.
    Sciflexarrayer Sx Microarray Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+sx+microarray+spotter/sciFLEXARRAYER+SX/10__1002_slash_adfm__202315035-450-19-23
    Average 95 stars, based on 1 article reviews
    sciflexarrayer sx microarray spotter - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.

    Journal: Advanced Functional Materials

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices

    doi: 10.1002/adfm.202315035

    Figure Lengend Snippet: Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.

    Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).

    Techniques: Confocal Microscopy, Cell Culture, Migration, Microarray, Concentration Assay, Co-Culture Assay